Enteric rice protein-shellac composite coating to enhance the viability of probiotic Lactobacillus salivarius NRRL B-30514

This study reports novel modified rice protein (MRP)-ammonium shellac (NH4SL) enteric composite coatings on millimeter-sized probiotics pellets to protect the survival of during storage, thermal treatments, and simulated gastrointestinal (GI) digestions. An aqueous MRP solution at pH 7.0–13.0 was dropwise added into an ethanol NH4SL 8.2, mixture significantly affected homogeneity MRP-NH4SL suspensions formed films. The MRPpH13-NH4SL suspension with 9.4 had smaller particles thus better stability than other ~8.4, predominantly due solubility a higher pH. Atomic force microscopy, fluorescence spectroscopy, dynamic light scattering results indicated complexation between in all which increased intermolecular repulsions further facilitate suspension. homogenous resulted smooth films improved mechanical properties content having pH-dependent solubility. Probiotics coated more viable Lactobacillus salivarius NRRL B-30514 uncoated after 30-day ambient heating 80 °C for 20 min, GI coating also preserved viability NH4SL-only 2-h gastric digestion. Therefore, MRPs can be used modify shellac-based edible deliver powdered probiotics, is significant manufacturing solid probiotics-containing products.